Effects of Dietary BioHerbal on Growth Performance, Newcastle Disease Antibody Response, and Growth-related Factor in Broiler Chickens

Abstract

The withdrawal of antibiotic growth promoters has intensified the need for effective phytogenic alternatives in broiler nutrition. This study evaluated the effects of dietary BioHerbal (2 g/kg feed) on growth performance, humoral immune response, hematological indices, serum biochemical parameters, and circulating growth factors in Ross 308 broiler chickens over a 42-day period. A total of 240 one-day-old male chicks were allocated to two dietary treatments with three replicate pens per treatment. BioHerbal supplementation significantly increased body weight gain and improved feed conversion ratio during both starter and overall growth phases (p<0.05). While primary antibody response to Newcastle disease vaccination was unaffected, secondary antibody titers at day 42 were significantly enhanced (p<0.05). Supplemented birds exhibited reduced serum glucose, triglycerides, and uric acid concentrations, along with increased globulin and calcium levels. Circulating IGF-1, TGF-β, VEGF, and EGF were significantly elevated (p<0.05), whereas growth hormone remained unchanged. These findings indicate that BioHerbal supplementation improves growth efficiency and is associated with increased secondary NDV antibody titers and changes in circulating growth-related factors